Tuesday, February 14, 2012
Thursday, February 9, 2012
Tarsiers have ultrasonic communication
Tuesday, February 7, 2012
New Nature Reserve Established in Peru
Read more:http://news.mongabay.com/2012/0207-hance_maijunareserve.html#ixzz1ljjtLJys
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Sunday, February 5, 2012
All in the Family, nature PORTUGAL
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Saturday, February 4, 2012
Tracking the mighty albatross - Environment Sci - Video - 3 News
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Snow Leopard Trust Featured on BBC World News!
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Friday, February 3, 2012
Grasshopper, PORTUGAL
Peneda-Gerês National Park, Portugal
July 2010
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Young Sally Lightfoot Crab, GALAPAGOS
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Thursday, February 2, 2012
Insect in a purple flower, PORTUGAL
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Wednesday, February 1, 2012
Ground-breaking research in the Amazon River
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Yellows and Browns in Gerês, Portugal
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Snow Leopards and Stem Cells
Induced pluripotency is a new approach to produce embryonic stem-like cells from somatic cells that provides a unique means to understand both pluripotency and lineage assignment. To investigate whether this technology could be applied to endangered species, where the limited availability of gametes makes production and research on embryonic stem cells difficult, we attempted generation of induced pluripotent stem (iPS) cells from snow leopard (Panthera uncia) fibroblasts by retroviral transfection with Moloney-based retroviral vectors (pMXs) encoding four factors (OCT4, SOX2, KLF4 and cMYC). This resulted in the formation of small colonies of cells, which could not be maintained beyond four passages (P4). However, addition of NANOG, to the transfection cocktail produced stable iPS cell colonies, which formed as early as D3. Colonies of cells were selected at D5 and expanded in vitro. The resulting cell line was positive for alkaline phosphatase (AP), OCT4, NANOG, and Stage-Specific embryonic Antigen-4 (SSEA-4) at P14. RT-PCR also confirmed that endogenous OCT4 and NANOG were expressed by snow leopard iPS cells from P4. All five human transgenes were transcribed at P4, but OCT4, SOX2 and NANOG transgenes were silenced as early as P14; therefore, reprogramming of the endogenous pluripotent genes had occurred. When injected into immune-deficient mice, snow leopard iPS cells formed teratomas containing tissues representative of the three germ layers. In conclusion, this was apparently the first derivation of iPS cells from the endangered snow leopard and the first report on induced pluripotency in felid species. Addition of NANOG to the reprogramming cocktail was essential for derivation of iPS lines in this felid. The iPS cells provided a unique source of pluripotent cells with utility in conservation through cryopreservation of genetics, as a source of reprogrammed donor cells for nuclear transfer or for directed differentiation to gametes in the future.
- R. Verma, M.K. Holland, P. Temple-Smith, P.J. Verma.Inducing pluripotency in somatic cells from the snow leopard (Panthera uncia), an endangered felid.Theriogenology, 2012; 77 (1): 220 DOI:10.1016/j.theriogenology.2011.09.022
- Monash University (2012, January 23). Saving the snow leopard with stem cells. ScienceDaily. Retrieved February 1, 2012, from http://www.sciencedaily.com/releases/2012/01/120123094758.htm
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Nature in Valais, Switzerland
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